Coding

Part:BBa_K4164001:Design

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-09-29)


Bile acid sulfate sulfatase（BSS）


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 918
    Illegal NgoMIV site found at 969
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for enhanced Escherichia coli expression.


Source

Pseudomonas testosteroni, sequence found through United States Patent No.5,585,255(1996).

References